Composite

Part:BBa_K3160011:Design

Designed by: chi xu   Group: iGEM19_XHD-WS-Wuhan-A   (2019-10-15)


pEGFP-miR196a-148a sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pEGFP- miR-196a miR-148a-sensor sensor was controlled by CMV promoter (CMV-GFP- miR-196a miR-148a). The plasmids of CMV-GFP- miR-196a miR-148a were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We also measuresd the fluorescence of GFP in different cells transfected with miR-196a miR-148a for 24 h by plate reader (SpectraMax i3), which analyses the potential value of CMV-GFP- miR-196a miR-148a to measure the expression of miR-196a miR-148a.


Source

We ordered this part DNA from a synthesis company



References